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KMID : 0380020040190020113
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 2 p.113 ~ p.117
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Effect of Ultrasonification Process on Enhancement of Immuno-stimulatory Activity of Ephedra sinica Stapf and Rubus coreanus Miq
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Park Jin-Hong
Lee Hyun-Soo
Mun Hyoung-Chul
Kim Dae-Ho
Seong Nak-Sul
Chung Hae-Gon
Bang Jin-Ki
Lee Hyeon-Yong
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Abstract
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The effect of ultrasonification on immuno-stimulatory activities of the extracts from Ephedra sinica Stapf and Rubus coreanus Miq. was observed by the growth of human immune cells. The extraction yields from water extracts and water extract with ultrasonification at 40, 60 and 100 were compared. The highest yield was observed enhancement ratio 2.34% in the extract at 40 with ultrasonification. The growth of human T-cell was increased up to 13.810 viable cells/ in adding 1.0 g/L of the ultrasonification extracts. The treated extracts increased the specific secretion of TNF- up to 10.510-4/ pg/cell. It was suggested that of the extracts on water the extraction yield with ultrasonification compared improved the extraction with ultrasonification of low than.
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KEYWORD
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Ephedra sinica, Rubus coreanus, ultrasonification, immuno-stimulatory, IL-6, TNF-α
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